Poor man's liquid nitrogen: dry ice and ethanol. At 100% the ethanol stays liquid and you can use it to flash-freeze cells in glycerol. I was freezing down some yeast stocks and I couldn't find the big thing of cheap ethanol (with the additive so you can't drink it) and I didn't want to use the expensive, pure stuff, so I figured 70% would be close enough.
2 comments:
why were you freezing ethanol?
Poor man's liquid nitrogen: dry ice and ethanol. At 100% the ethanol stays liquid and you can use it to flash-freeze cells in glycerol. I was freezing down some yeast stocks and I couldn't find the big thing of cheap ethanol (with the additive so you can't drink it) and I didn't want to use the expensive, pure stuff, so I figured 70% would be close enough.
It wasn't, but it ended up working okay anyway.
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